Objective: To establish a new accurate method for the determination of mineral oil saturated hydrocarbon （MOSH） in infant formula by solid phase extraction combined with gas chromatography-Flame Ionization Detection （SPE-GC-FID）. Methods: Different extraction methods such as soaking extraction, oscillating extraction, ultrasonic assisted extraction, liquid-liquid extraction were used to extract MOSH from infant formula milk powder, and different extraction solvents such as n-hexane, isooctane, benzene, mixed solvents were used to extract MOSH. The optimal extraction method and extraction solvent were determined based on the extraction amount of MOSH. The target compounds purified by AgNO₃ SPE column, and finally detected by gas chromatography-Flame Ionization Detection （GC-FID）. Results: The calibration curve of MOSH was linear in the range of 10.0～1 000 μg/mL with correlation coefficient of 0.999 97. The limit of detection was 1.0 mg/kg, and the limit of quantitation was 3.0 mg/kg. The recoveries from spiked samples were 93.3%～103.7%, and the relative standard deviations were 2.3%～5.9%. The MOSH in 8 commercially available infant formula was detected by the established method, and the results were between 1.06 and 9.83 mg/kg, indicating that there was a certain risk of MOSH contamination in infant formula. Conclusion: This method is accurate, sensitive, and suitable for the detection of MOSH in infant formula.