Objective: This study aimed to investigate the effect of Eurotium Cristatum fermentation broth mixed with tea polyphenols on the immune indexes of cyclophosphamide immunosuppressive mice. Methods: Immunosuppressive model was established by intraperitoneal injection of cyclophosphamide with 80 mg/kg for 3 days. High, middle and low dose E. Cristatum fermentation broth mixed with tea polyphenols was administrated to immunosuppressive mice intragastrically for 15 days. The blood index, spleen index, macrophage phagocytic index, half hemolysis value, delayed-type hypersensitivity, the content of IL-2 and tumor necrosis factor-α (TNF-α) were measured. Results: E. Cristatum fermentation broth mixed with tea polyphenols significantly increased the number of white blood cells, red blood cells, hemoglobin, serum hemolysin and IL-2 on immunosuppressive mice, enhanced the spleen index and monocyte-phagocytic index, and improved the delayed type hypersensitivity on mice. Conclusion: E. Cristatum fermentation broth mixed with tea polyphenols can enhance immunity on mice.