Objective： To study the effect of hemp seed and algal oil mixture in lowering blood lipids, and provide the theoretical basis for the utilization of hemp seed and algal oil mixture in food. Methods: 113 hyperlipidemia adult volunteers were selected and randomly divided into two groups, namely the experiment group and the control group. The subjects in the experiment group were given gelatinized confections for 90 days, while subjects in the control group were given a placebo. The levels of Serum total cholesterol(TC), Triglyceride(TG), High-density lipoprotein cholesterol(HDL-C), Low-density lipoprotein cholesterol(LDL-C)and physiological and biochemical parameters were measured before and after trial. For the experiment group, hepatobiliary ultrasonography, carotid plaque ultrasonography, fasting blood glucose, glycosylated hemoglobin, inflammatory indicators tumor necrosis factor-α, interleukin-6, C-reactive protein and antioxidant indicators superoxide dismutase, malondialdehyde, glutathione peroxidase were detected. Results: At the end of the human trial, compared with the control group, serum TC, TG and LDL-C levels of the experiment group decreased significantly(P<0.05). And the rate of effective lowering lipid level is 30.4％ in the experiment group at the end of trials. No abnormal effect was observed in liver function, renal function and hematological examination. In the experiment group, the levels of hBA1c and IL-6 were decreased (P<0.05), SOD and GSH-Px activities increased (P<0.01), the improvement rate of carotid plaque and fatty liver was 33.3% and 39.3%, respectively. Conclusion: Hemp seed and algal oil mixture tested in the study have a good function of lowering blood lipid in humans with an acceptable safety profile, which may be related to anti-inflammatory and antioxidant effects.