Objective: To established a method for determination of 21 perfluorinated compounds in animal-derived food by ultra-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. Methods: The sample was extracted with 1% formic acid-acetonitrile, cleaned up with a sorbent mixture of N-propylethylenediamine （PSA）, octadecyl bonded silica gel （C₁₈） and graphitized carbon black （GCB）, then separated by acetonitrile-5mmol/L ammonium acetate aqueous solution and Acquity UPLC^ BEH C₁₈, detected by liquid chromatography tandem mass spectrometry in negative mode using the multiple reaction monitoring （MRM） mode. Results: The calibration curves were linear in the range of 0.2~10 ng/mL for the 21 PFAS. The limits of quantification were 0.25 μg/kg. Average recoveries were in the range of 71%~118% with three spiked levels, namely 0.25, 0.50, 1.25 μg/kg. The relative standard deviations （RSDs%） of inter-batch and intra-batch were 3.5%~18.3% （n=6） and 2.2%~13.8% （n=3）, respectively. Conclusion: The simple pretreatment and high sensitivity satisfied the fast and accurate detection requirement, meanwhile it is suitable for qualitative and quantitative analysis of perfluorinated compounds in animal-derived food.