浊点萃取—异辛烷反萃取—气相色谱质谱法测定茶饮料中8种农药残留
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(1. 无锡市疾病预防控制中心,江苏 无锡 214023;2. 南京医科大学,江苏 南京 211166)

作者简介:

谢洁,女,无锡市疾病预防控制中心副主任技师,学士。

通讯作者:

王宇(1970—),女,无锡市疾病预防控制中心高级实验师,学士。E-mail:wy701210@126.com

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基金项目:

无锡市卫生健康委员会医学创新团队项目(编号:CXTD2021004)


Cloud point extraction coupled with Isooctane back extraction for determination of eight pesticide residues in tea beverages by gas chromatography mass spectrometry
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(1. Wuxi Center for Disease Control and Prevention, Wuxi, Jiangsu 214023, China; 2. Nanjing Medical University, Nanjing, Jiangsu 211166, China)

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    摘要:

    目的:快速测定茶饮料中有机氯和拟除虫菊酯类农药残留量。方法:选用非离子表面活性剂聚乙二醇4000(PEG-4000)作为浊点萃取剂,用异辛烷超声反萃取后,取上层异辛烷溶液上机。采用HP-5 ms UI毛细管色谱柱对异辛烷溶液进行分离,再经GC/MS进行定性和定量分析。结果:有机氯和拟除虫菊酯类农药分别在0.01~2.0,0.02~4.0 mg/L的质量浓度范围内呈良好线性关系,相关系数均大于0.995;方法的检出限为0.003~0.008 mg/kg,定量限为0.01~0.02 mg/kg,平均回收率78.3%~98.4%,相对标准偏差2.7%~8.5%(n=6)。结论:该方法操作过程简单,富集率高、实用、安全、经济,适用于茶饮料类液体饮料中的农药多残留的快速测定。

    Abstract:

    Objective: Rapid determination of organochlorine and pyrethroid pesticide residues in tea beverages. Methods: Selecting the non-ionic surfactant PEG-4000 as cloud point extraction, pesticides were extracted with isooctane by ultrasonic back extraction. The upper layer of isoctane solution was analyzed by Gas Chromatography/Mass. The isooctane solution was separated by HP-5 ms UI capillary column and analyzed qualitatively and quantitatively by GC/MS. Results: The eight pesticides showed a good linear relationship between 0.01~2.0 and 0.02~4.0 mg/L, respectively, with the correlation coefficients (R) of over 0.995. The detection limit and quantification limit of this method were 0.003~0.008 mg/L and 0.01~0.02 mg/L, respectively. The average recovery was 78.3%~98.4%, RSD was 2.7%~8.5% (n=6). Conclusion: The method is simple in operation, high in enrichment rate, practical, safe and economical, which is suitable for rapid determination of pesticide residues in tea drinks.

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谢 洁,周 闰,孔令灿,等.浊点萃取—异辛烷反萃取—气相色谱质谱法测定茶饮料中8种农药残留[J].食品与机械,2023,39(1):31-36,156.
XIE Jie, ZHOU Run, KONG Ling-can, et al. Cloud point extraction coupled with Isooctane back extraction for determination of eight pesticide residues in tea beverages by gas chromatography mass spectrometry[J]. Food & Machinery,2023,39(1):31-36,156.

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  • 收稿日期:2022-10-10
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  • 在线发布日期: 2023-04-25
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