Objective：Thisstudy aimedto investigatethedifference of bio activators from different flaxseed .Methods： 12flaxseed varieties were used as study objectives， 8kindsofbioac-tivat ors from different varieties of flaxseed were measured andanalyzed by principal component and clustering.Results：Theorder of effect of flaxseed varieties on bioactive substances was asfollows：lignan＞total cyanide＞flavone＞phytosterol＞total phe-no lic acid＞to cop hero nic acid＞FRAP value＞DP PH value.3 principal components were extracted by principal component a-nalysis and their accumulative contribution ratereach to82.925%.The first， second and third principal component weremainly determined by flavone and FRAP values， lignan and to co-p hero nic acid， total cyanide and total phenolic acid， respectively.12 flaxseed varieties were divided into three groups by clusteringanalysis， and their comprehensive assessment value reduced suc-ces sively.Group I include Bay all， N in gyal 7， Bay a 9， Long ya 8and Lun xuan 2，Group II include Long ya10，N in gyal 6 andNingyal6andJinya 7， and Group III include Lun xuan l， Jin ya 8，Yiya3 and Yiya 4.Conclusion：Comprehensive score of Bay all isthe highest， and it has higher nutritional value.