Objective：This study aimed to develop R.roxburghii as functional food in medicine and healthcare products.Methods：High-fat dial-induced insulin resistance rat model was established.There were normal control group， the model group， the positivecontrol group， and the low and high dose group of R.roburghii polysaccharide(100mg/kg and 200mg/kg) .The body weight was recorded before and after the experiment.The serum oxidative stress indexes(SOD， MDA) ， lipid metabolism indexes(TG， TC) ， fasting blood glucose(FBG) and fasting insulin(FINS) levels， as well as the mRNA and protein expressions of PI3K an dG LUT 4m RNA in skeletal muscle were detected.Re-sults：Compared with the model group， the bodyweight， serum MDA， TG， TC， FBG， FINS， HOMA-IR were decreased(P＜0.0l) ， and the SOD activity and the m RNA and protein expres-sion levels of PI3KandGLUT 4 were increased after poly sac cha-ride intervention in IR rats after R.roxburghii polysaccharide in-ter vention(P＜0.05) .Conclusion：R.roc burghii polysaccharide(PRR-3) has antioxidant effect， which can reduce the weight ofinsulin-resistant rats， regulate glucose and lipid metabolism， andimprove obesity insulin resistance.There is a dose-effect relation-ship， which maybe achieved by up-regulating the expression of PI3KandGLUT 4 proteins in the PI3K/AKT/GLUT 4 signalingpathway and enhancing GLUT 4 transport.