QuEChERs-同位素内标—气相色谱—串联质谱法快速测定茶叶中4种糠醛类物质
CSTR:
作者:
作者单位:

作者简介:

刘粲迪,男,湖南师范大学在读硕士研究生。

通讯作者:

中图分类号:

基金项目:

湖南省重点研发计划项目(编号:2020SK2092);国家自然科学基金项目(编号:21775040,21775051)


Determination furfurals in tea products by QuEChERs-Isotope IS-GC-MS/MS
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:检测茶叶中糠醛类物质。方法:建立了同时测定茶叶中糠醛、5-甲基糠醛、5-羟甲基糠醛和2-乙酰呋喃的气相色谱—串联质谱法(GC-MS/MS),对样品的前处理方法及GC-MS/MS方法进行了优化。采用糠醛-D4作为内标,样品经过乙腈提取,QuEChERs法进行处理净化,GC-MS/MS多反应监测(MRM)进行测定。结果:4种糠醛类物质在1.25~200.00 μg/L的质量浓度范围内均呈良好的线性关系,相关系数≥0.999 0,方法检出限(S/N=3)均为1 μg/kg,加标回收率范围在89.10%~113.02%。经过对市售茶叶的检测,大部分都检出含有糠醛类物质。结论:该方法简便、快速,可以消除茶叶中复杂基质的干扰,结果准确可靠、灵敏度高,适用于茶叶中4种糠醛类物质的同时测定。

    Abstract:

    Objective: This study aimed to establish a new method for detecting furfurals in tea. Methods: A gas chromatography tandem mass spectrometry (GC-MS/MS) method was established for the simultaneous determination of furfural, 5-methylfurfural, 5-hydroxymethylfurfural and 2-Acetylfuran in tea. The sample pretreatment method and GC-MS/MS method were optimized. Furfural-D4was used as the internal standard. The sample was extracted by acetonitrile, treated and purified by QuEChERS method, and determined by GC-MS/MS multi reaction monitoring (MRM). Results: The four furfurals showed good linear relationship in the mass concentration range of 1.25~200.00 μg/L, with the correlation coefficient ≥0.999 0, and the detection limit (S/N=3) was 1 μg/kg, with the recovery range of standard addition of 89.10%~113.02%. Most of the commercially available tea samples were found to contain furfural. Conclusion: This method in the present study is simple, rapid and can eliminate the interference of complex matrix in tea. The result is accurate, reliable and sensitive. It is suitable for the simultaneous determination of four furfurals in tea.

    参考文献
    相似文献
    引证文献
引用本文

刘粲迪,陈波,尹芳平,等. QuEChERs-同位素内标—气相色谱—串联质谱法快速测定茶叶中4种糠醛类物质[J].食品与机械,2022,(5):30-36.
LIU Can-di, CHEN Bo, YIN Fang-ping, et al. Determination furfurals in tea products by QuEChERs-Isotope IS-GC-MS/MS[J]. Food & Machinery,2022,(5):30-36.

复制
相关视频

分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2022-06-30
  • 出版日期:
文章二维码
×
《食品与机械》
友情提示
友情提示 一、 近日有不少作者反应我刊官网无法打开,是因为我刊网站正在升级,旧网站仍在百度搜索排名前列。请认准《食品与机械》唯一官方网址:http://www.ifoodmm.com/spyjx/home 唯一官方邮箱:foodmm@ifoodmm.com; 联系电话:0731-85258200,希望广大读者和作者仔细甄别。