Objective:To make up for the lack of research on the extraction technology, fatty acid composition, and physical and chemical properties of Canavalia gladiata oil in China, and provide technical support for the development and utilization of Canavalia gladiata oil.Methods:The process parameters of extracting Canavalia gladiata oil with n-hexane were optimized. Gas chromatography-mass spectrometry (GC-MS) was used to analyze and determine the type and content of fatty acids in Canavalia gladiata oil.Results:The best extraction conditions for the Canavalia gladiata oil were as follows: the ratio of liquid-to-material was 25∶1 (mL/g), the temperature for extracting was 70 ℃, the time for extracting was 2 h. Under these extraction conditions, the extraction rate of the Canavalia gladiata oil was 3.12%. 9 kinds of fatty acids were analyzed and determined by GC-MS method, of which 3 kinds of fatty acids were mainly palmitic acid, oleic acid and linoleic acid, which accounted for about 85.29% of the total fatty acids, and unsaturated fatty acids were 89.12%. The content of saturated fatty acid was 10.88%, the content of monounsaturated fatty acid was 72.53%, the content of diunsaturated fatty acid was 12.76%, and the content of triunsaturated fatty acid was 3.83%.Conclusion:Canavalia gladiata oil was dominated by unsaturated fatty acids, oleic acid content was the highest, The types of fatty acids were similar to soybean oil, but the ratio was different, and the physical and chemical properties were similar to common peanut oil and soybean oil.