Objective: To investigate the effect of storage time on the quality of paper-making reconstituted tobacco, the change of internal chemical components, tensile strength and thermogravimetric properties of paper-making reconstituted tobacco were studied. Methods: Continuous flow analyzer, tensile strength tester and thermogravimetric analyzer were introduced to analyze three kinds of paper-making reconstituted tobacco with different storage time. Results: The contents of total alkaloids, total sugar, total nitrogen and moisture of JS-A and JS-B samples changed significantly with the increase of storage time. While the content of total alkaloids and total nitrogen of JS-C samples didnt change much. Besides, storage time had a significant effect on the longitudinal tensile strength of the three kinds of reconstituted tobacco leaves. Further, for all of the samples, the maximum values appeared at 180 days. The weight loss rate and normalized enthalpy of the three samples in the second stage changed significantly with the increase of storage time, and reached the maximum value at 360 days. Conclusion:The suitable storage time of three kinds of reconstituted tobacco leaves was between 180 and 360 days.