芸豆/大豆复合发酵液代谢组分及功能性研究
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(1. 黑龙江八一农垦大学食品学院,黑龙江 大庆 163319;2. 国家杂粮工程技术研究中心,黑龙江 大庆 163319;3. 粮食副产物加工与利用教育部工程研究中心,黑龙江 大庆 163319;4. 黑龙江省农产品加工与质量安全重点实验室,黑龙江 大庆 163319)

作者简介:

李志芳,女,黑龙江八一农垦大学在读硕士研究生

通讯作者:

王颖(1979—),女,黑龙江八一农垦大学教授,博士。E-mail: wychen156@163.com

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基金项目:

国家重点研发计划项目(编号:2018YFE0206300);黑龙江省自然科学基金研究团队项目(编号:TD2020C003);黑龙江省“杂粮生产与加工”优势特色学科资助项目(编号:黑教联\[2018\]4号);黑龙江八一农垦大学“三横三纵”科研团队支持计划(编号:TDJH201806)


Studies on metabolites and functions of soy-based plant fermentation broth
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(1. College of Food, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China; 2. National Coarse Cereals Engineering Research Center, Daqing, Heilongjiang 163319, China; 3. Engineering Research Center of Processing and Utilization of Grain By-products, Ministry of Education, Daqing, Heilongjiang 163319, China; 4. Key Laboratory of Agro-Products Processing and Quality Safety of Heilongjiang province, Daqing, Heilongjiang 163319, China)

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    摘要:

    目的:探究豆基植物发酵液在发酵过程中代谢产物及抗氧化活性的变化规律。方法:以紫花芸豆、大豆为原料,采用酵母菌、乳酸菌等多种益生菌共同发酵制备复合发酵液,并对发酵过程中pH值、总酸、还原糖、可溶性固形物、总酚、黄酮含量及抗氧化活性进行分析。结果:发酵液pH值随发酵时间的延长不断降低并逐步稳定在3.55左右,总酸由0.13 mg/mL上升至0.43 mg/mL,可溶性固形物由10.01%降低至5.73%,还原糖含量在发酵过程中呈先下降后保持波动平衡的状态,总酚、黄酮类物质含量在发酵过程中均先迅速上升,后分别稳定在430 mg/mL和155.30 μg/mL左右,发酵液还原力、ABTS自由基清除能力总体呈持续增长趋势并于发酵后期达稳定状态,分别于发酵48 h时达到最大值(0.608,3.404 mmol/L)。结论:芸豆/大豆复合发酵液常规代谢组分的变化趋势存在一定规律性,且该发酵液具有一定的抗氧化能力。

    Abstract:

    Objective: To explore the changes of metabolites and antioxidant activity of soybean based plant fermentation broth during fermentation. Methods: The experiment was conducted to prepare the compound fermentation liquid from kidney beans and soybean by fermentation of yeast, Lactobacillus and other probiotics, The pH value, total acid, reducing sugar, soluble solids, total phenol, flavone content and antioxidant activity in the fermentation process were analyzed. Results: The pH value of fermentation broth decreased with the extension of fermentation time and gradually stabilized at about 3.55, the total acid content increased from 0.13 mg/mL to 0.43 mg/mL, and the soluble solid content decreased from 10.01% to 5.73%. The content of reducing sugar decreased at first and then kept fluctuating balance. The total phenol content increased rapidly in the fermentation process, and then stabilized at about 430 mg/mL. The content of flavonoids also increased with the fermentation, and then stabilized at 155.30 μg/mL. The reducing power and ABTS free radical scavenging capacity of fermentation broth showed a continuous growth trend, and reached a stable state in the late stage of fermentation, reaching the maximum value (0.608 and 3.404 mmol/L) at 48 h of fermentation, respectively. Conclusion: There is a certain regularity in the change trend of conventional metabolic components in kidney bean and soybean compound fermentation broth, and the fermentation broth has a certain antioxidant capacity.

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李志芳,佐兆杭,王颖,等.芸豆/大豆复合发酵液代谢组分及功能性研究[J].食品与机械,2021,37(11):38-43.
LIZhifang, ZUOZhaohang, WANGYing, et al. Studies on metabolites and functions of soy-based plant fermentation broth[J]. Food & Machinery,2021,37(11):38-43.

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  • 收稿日期:2021-03-10
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  • 在线发布日期: 2023-02-15
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