Objective: The extraction process optimization study of dihydromyricetin in the combination of medicine and food homologous materials （Ampelopsis grossedentata 30%, mulberry leaf 15%, chrysanthemum 15%, reed root 10%, malt 10%, licorice 10% and light bamboo leaf 10%） was carried out to optimize its extraction process, in order to provide a scientific and theoretical reference for the development of medicine and food homologous materials and the production of extract powder. Methods: The times extractions was firstly investigated, and then the soaking time, water addition and extraction time were used as influencing factors to optimize the conditions for the extraction of dihydromyricetin from the combination of medicinal and food ingredients by aqueous extraction using L9 （3⁴） orthogonal table based on a single-factor test. Results: The amount of water added had a significant effect （P<0.05） on the extraction of dihydromyricetin and the yield of infusion in the combination of medicinal and food ingredients. Considering the cost, timeliness and stability of the production, the optimal conditions for the water extraction process were 0.5 h of soaking with water with twice extractions, and the first extraction with the solution including 10 times water of its mass for 1.5 h and the second with 8 times water of its mass for 1.0 h. Under the control of these optimal conditions, the amount of dihydromyricetin extracted from 65 g of the combination of medicine and food homologous materials was 3 761.14 mg, and the yield of infusion was 31.42%. Conclusion: The hot water reflux extraction method can be used as an extraction method for the combination of medicinal and food homologous materials. This method is simple, feasible, efficient, and accurate. It can be used to optimize the extraction process of dihydromyricetin in the combination of medicinal and edible materials.