基于QuEChERS的UHPLC-MS/MS同时检测鸡蛋中19种农药残留量
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(长沙环境保护职业技术学院,湖南 长沙 410004)

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黄小波(1972—),男,长沙环境保护职业技术学院高级工程师,硕士。E-mail:444815857@qq.com

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湖南省自科基金科教联合项目(编号:2017JJ5060)


Simultaneous determination of 19 kinds of pesticide residues in eggs by UHPLC-MS/MS based on QuEChERS
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(Changsha Environmental Protection Vocational and Technical College, Changsha, Hunan 410004, China)

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    摘要:

    目的:构建一种同时、快速、高效的检测鸡蛋中多种农残方法。方法:将QuEChERS与UHPLC-MS/MS结合用于鸡蛋中19种农药残留量的检测。结果:所建方法的检出限(LOD)和定量限(LOQ)分别为(0.39±0.66),(1.28±2.17) μg/kg;加标回收率为79.25%~102.14%,相对标准偏差均值(RSD)为9.34%~16.72%;除恶唑菌酮外,鸡蛋中其余18种农药的线性相关系数接近0.999。所测鸡蛋中鸡蛋中19种农残检出总频率为2.82%,农残问题主要涉及辛硫磷和毒死蜱,其检出频率分别为22%,15%,浓度范围分别为2.08~729.74,0.81~46.89 μg/kg。结论:所建方法准确可靠,符合相关检测要求;湖北省境内鸡蛋中农残检出频次低、安全隐患较小。

    Abstract:

    Objective: The aim is to establish a rapid, efficient and simultaneous detection method for various pesticide residues in eggs. Methods: QuEChERS combined with UHPLC-MS/MS was successfully used to detect 19 kinds of pesticide residues in eggs. Results: The limit of detection (LOD) and quantification (LOQ) of this method were (0.39±0.66) and (1.28±2.17) μg/kg, respectively. The recoveries of standard pesticides and relative standard deviations ranged from 79.25% to 102.14% and from 9.34% to 16.72%, respectively. Except for famoxadone, the correlation coefficients of linear equations for all pesticides were close to 0.999. The total frequency of 19 kinds of pesticides detected in eggs was 2.82% and the detection frequency of chlorpyrifos and phoxim were 22% and 15%, which maybe the potential safety issue for eggs. The quantity of them ranged from 2.08~729.74 μg/kg and 0.81~46.89 μg/kg, respectively. Conclusion: The proposed method is accurate, reliable, and meets the requirements for testing pesticides in eggs. The detection frequency of pesticides in eggs in Hubei province is low, indicating a low safety risk.

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黄小波,刘维平.基于QuEChERS的UHPLC-MS/MS同时检测鸡蛋中19种农药残留量[J].食品与机械,2021,37(10):56-60.
HUANGXiaobo, LIUWeiping. Simultaneous determination of 19 kinds of pesticide residues in eggs by UHPLC-MS/MS based on QuEChERS[J]. Food & Machinery,2021,37(10):56-60.

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  • 收稿日期:2021-07-10
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  • 在线发布日期: 2023-02-15
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