基于GC/MS结合多元统计学筛选爆珠香精中香味标志物
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(江苏中烟工业有限责任公司技术中心,江苏 南京 210019)

作者简介:

张华,女,江苏中烟工业有限责任公司工程师,硕士。

通讯作者:

廖惠云(1979—),男,江苏中烟工业有限责任公司高级工程师,硕士。E-mail: liaohy@jszygs.com

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江苏中烟工业有限责任公司科技创新项目(编号:201703,201802)


Identification of characteristic flavor markers in breakable capsules for flavoring by GC/MS combined with multivariate statistical analysis
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(Technology Center, China Tobacco Jiangsu Industrial Co., Ltd., Nanjing, Jiangsu 210019, China)

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    摘要:

    目的:筛选出爆珠香精质量控制用的特征香味组分标志物。方法:采用气相色谱—质谱法(GC/MS)对爆珠香精中挥发性及半挥发性化合物进行检测,建立目标样品色谱指纹图谱,并对组分数据进行主成分分析(PCA)和正交偏最小二乘法—判别分析(OPLS-DA)。结果:GC/MS共鉴定出27种挥发性、半挥发性物质,包含柠檬烯、丁香酚、茴香脑、薄荷醇等香味化合物以及辛癸酸甘油混合酯壁材物质;优选条件下的仪器精密度、日内精密度和日间精密度分别为0.56%~9.94%,3.07%~9.81%,2.81%~11.95%,结合ChemPattern软件近邻算法对图谱数据进行对齐,建立了爆珠香精样品的GC/MS指纹图谱;PCA分析对正常与模拟状态下爆珠香精样品区分不明显,但基于OPLS-DA分析发现共有4种化合物被鉴定为特征标志物(变量重要性投影值>1,P<0.05),分别为柠檬烯、辛酸甲酯、癸酸甲酯和丁香酚,该系列特征物质可反映爆珠香精果香香韵的特点。结论:使用GC/MS采集样品指纹图谱,结合OPLS-DA分析手段,能够有效筛选出爆珠香精特征香味的标志物,为客观评价爆珠香精质量提供参考。

    Abstract:

    Objective: Identify the characteristic markers for the quality control of breakable capsule flavor. Methods: Volatile and semi-volatile compounds were detected by GC/MS, and the fingerprinting of the target samples was established. The obtained data were analyzed by multivariate statistical analysis. Results: 27 kinds of volatile and semi-volatile substances were identified, including 20 dominant aroma compounds and 7 shell materials, which were limonene, eugenol, anethole, menthol and so on, and caprylic/capric triglyceride respectively. Under the optimized conditions, the instrument precision, intra-day precision and inter-day precision were between 0.56%~9.94%, 3.07%~9.81% and 2.81%~11.95% respectively. Combined with the nearest neighbor algorithm of ChemPattern software, GC/MS fingerprinting of the sample was established. PCA showed there were no significant difference between the normal and simulated breakable capsule flavor samples. However, based on the results of OPLS-DA, four compounds were identified as characteristic markers (variable importance projection value>1, P<0.05), which were limonene, methyl octanoate, methyl decanoate and eugenol, respectively. In general, these characteristic substances can reflect the fragrant type of breakable capsule flavor. Conclusion: This study can provide a reference for the quality control of breakable capsule flavor.

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张华,刘琪,叶远青,等.基于GC/MS结合多元统计学筛选爆珠香精中香味标志物[J].食品与机械,2021,(9):37-43.
ZHANGHua, LIUQi, YEYuanqing, et al. Identification of characteristic flavor markers in breakable capsules for flavoring by GC/MS combined with multivariate statistical analysis[J]. Food & Machinery,2021,(9):37-43.

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  • 收稿日期:2021-04-19
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  • 在线发布日期: 2023-02-15
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