酱卤肉制品中食品添加剂的固相提取与精确分析
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(1. 烟台理工学院食品与生物工程系,山东 烟台 264005;2. 山东理工大学农业工程与食品科学学院,山东 淄博 255000)

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王晓君(1979—),女,烟台理工学院讲师,硕士。E-mail:wsj1290@126.com

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山东省高校自然科学研究项目(编号:19KSDB460041)


Solid-phase extraction and accurate analysis on food additives in marinated meat products
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(1. Department of Food and Biological Engineering, Yantai University of Technology, Yantai, Shandong 264005, China; 2. School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong 255000, China)

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    摘要:

    目的:建立酱卤肉制品中苯甲酸、山梨酸和糖精钠的精确定量方法。方法:样品经乙酸乙酯萃取,SPE-MCX固相萃取处理,采用高效液相色谱(High Performance Liquid Chromatography,HPLC)检测。色谱柱为Agilent ZORBAX Eclipse XDB-C18(250 mm×4.6 mm,5 μm),流动相为75% 0.02 mol/L乙酸铵水溶液和25%乙腈混合溶液,柱温为25 ℃,流速0.8 mL/min,进样量10 μL;检测波长230 nm。结果:3种添加剂质量浓度在0.5~10.0 mg/L范围内,其峰面积与质量浓度呈良好的线性关系,R2>0.990,且3种添加剂检测限均低于3.52×10-2 mg/kg,回收率为95.44%~101.24%,相对标准偏差均小于2.92%。结论:该方法简单、灵敏、快速、准确,适用于酱卤肉制品中苯甲酸、山梨酸和糖精钠3种添加剂的日常检测。

    Abstract:

    Objective: To establish an accurate method for the determination of benzoic acid, sorbic acid and saccharin sodium in soy sauce brine meat products. Methods: The samples were extracted by ethyl acetate, SPE-MCX solid phase extraction, and detected by HPLC simultaneously. Results: The determination was performed on Agilent Zorbax Eclipse XDB-C18 column (250 mm×4.6 mm, 5 μm) with mobile phase consisting of 75% 0.02 mol/L ammonium acetate solution and 25% acetonitrile. The column temperature was set at 25 ℃, and the flow rate was 0.8 mL/min. The sample size was 10 μL. The detection wavelength was 230 nm. In the range of 0.5 ~ 10.0 mg/L, the peak area of the three additives showed a good linear relationship with the mass concentration (R2 > 0.990). The detection limits of the three additives were all lower than 3.52×10-2 mg/kg, the recoveries were 95.44% ~ 101.24%, and the relative standard deviations were all less than 2.92%. Conclusion: The method is simple, sensitive, rapid and accurate. It is suitable for the routine detection of benzoic acid, sorbic acid and saccharin sodium in soy sauce brine meat products.

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王晓君,李凤玲.酱卤肉制品中食品添加剂的固相提取与精确分析[J].食品与机械,2021,37(8):94-99.
WANGXiaojun, LIFengling. Solid-phase extraction and accurate analysis on food additives in marinated meat products[J]. Food & Machinery,2021,37(8):94-99.

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  • 收稿日期:2021-04-27
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  • 在线发布日期: 2023-02-15
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