Objective: A rapid method for simultaneous determination of 18 quinolones residues in raw milk was established. Methods: The raw milk was pretreated by liquid-liquid extraction, and the residues of 18 quinolones in raw milk were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry. Results: The linearity of all the 18 quinolones from 2.0 to 100.0 ng/mL had correlation coefficients greater than 0.992. The limits of detection (LOD) of this method ranged from 0.02 to 0.50 μg/kg, and the limits of quantification (LOQ) were 0.06 to 1.50 μg/kg. The average recoveries of the 18 quinolones were 74.0% to 106.5%, with the relative standard deviation （n=6） of 1.1%~13.0%. Conclusion: The method is simple, efficient and accurate, and can be used for the quantitative confirmation of quinolones residues in raw milk.