铁皮石斛茎、叶、花功能性成分、抗氧化活性及其相关性
CSTR:
作者:
作者单位:

(1. 铜仁职业技术学院,贵州 铜仁 554300;2. 甘肃农业大学农学院,甘肃 兰州 730070)

作者简介:

唐文文,女,铜仁职业技术学院副教授,甘肃农业大学在读博士研究生。

通讯作者:

陈垣(1963—),男,甘肃农业大学教授,博士。E-mail:chenyuan@gsau.edu.cn

中图分类号:

基金项目:

教育部精准扶贫项目(编号:XZ20190326);铜仁市科技计划项目(编号:铜市科研\[2020\]99号);贵州省职业院校石斛产业专业建设项目(编号:黔教办职成\[2019\]35号);甘肃省现代农业中药材产业体系首席专家(编号:GARS-ZYC-1)


Analysis of functional composition、 antioxidant activity and their correlation in stem, leaf and flower from Dendrobium officinale
Author:
Affiliation:

(1. Tongren Polytechnic College, Tongren, Guizhou 554300, China;2. College of Agriculture, Gansu Agricultural University, Lanzhou, Gansu 730070, China)

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探索铁皮石斛茎、叶、花主要功能性成分的差异性、抗氧化能力大小及抗氧化活性的主要贡献成分。方法:以铁皮石斛茎、叶、花为试验材料,分别采用苯酚—硫酸法、NaNO2-Al3+NaOH法、福林酚法和酸性染料比色法对其多糖、总黄酮、总多酚和总生物碱含量进行测定比较,研究茎、叶、花的抗氧化能力,并通过相关性分析确定其主要抗氧化活性成分。结果:铁皮石斛茎、叶、花中均含有多糖、黄酮、多酚和生物碱类成分,但含量差异极显著(P<0.01),其中多糖含量为茎(31.00%)>叶(15.04%)>花(8.11%);总黄酮和总多酚含量均以花中最高,分别为2.133%和15.71%,叶中次之;总生物碱含量以花中最高,达0.022 3%,为茎中的近4倍;铁皮石斛茎、叶、花均具有较高抗氧化活性,且随着浓度的增大,其对DPPH自由基和ABTS自由基的清除能力逐渐增强,其中花的水提物和醇提物清除DPPH自由基和ABTS自由基的IC50均最小,说明其氧化活性最大,较接近于维生素C的。相关性分析发现,铁皮石斛茎、叶、花抗氧化活性与总黄酮、总多酚和总生物碱含量呈正相关,与多糖含量无明显相关性,说明总黄酮和总多酚含量对铁皮石斛茎、叶、花的抗氧化活性贡献最大,总生物碱含量的贡献显著。结论:铁皮石斛叶和花同样具有广阔的应用价值和开发前景。

    Abstract:

    Objective: To strengthen the comprehensive utilization of Dendrobium officinale(D. officinale) resources. Methods: The functional composition, antioxidant activity and their pearson correlation analysis of D. officinale stem, leaf and flower were studied and compared. Results: The results showed that the contents of 4 main functional components and antioxidant activity were significantly different between the stem, leaf and flower from D. officinale, the polysaccharide of leaf and flower were lower than that observed in stem: Stem(31.00%)>Leaf(15.04%)>Flower(8.11%); However, compared with stem, the total flavonoid, total polyphenol and total alkaloids contents of leaf and flower were much higher. The results of DPPH, ABTS, FRAP analyses all indicated that the D. officinale stem, leaf and flower all exhibited a great antioxidant activities, the flower approached that of vitamin C (VC) at specific concentrations, the alcohol extract of leaf exhibited a higher antioxidant capacity than stem, The antioxidant activity of the samples increased positively with the concentration. According to correlation analysis, the total phenols and flavonoids have the strongest contribution to the antioxidant capacity(P<0.01), total alkaloid has stronger contribution to the antioxidant capacity(P<0.05). Conclusion: This study provided guidance for the comprehensive development and utilization of D. officinale.

    参考文献
    相似文献
    引证文献
引用本文

唐文文,夏俊丽,陈垣.铁皮石斛茎、叶、花功能性成分、抗氧化活性及其相关性[J].食品与机械,2021,37(7):45-50.
TANGWenwen, XIAJunli, CHENYuan. Analysis of functional composition、 antioxidant activity and their correlation in stem, leaf and flower from Dendrobium officinale[J]. Food & Machinery,2021,37(7):45-50.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2021-04-03
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2023-02-15
  • 出版日期:
文章二维码
×
《食品与机械》
友情提示
友情提示 一、 近日有不少作者反应我刊官网无法打开,是因为我刊网站正在升级,旧网站仍在百度搜索排名前列。请认准《食品与机械》唯一官方网址:http://www.ifoodmm.com/spyjx/home 唯一官方邮箱:foodmm@ifoodmm.com; 联系电话:0731-85258200,希望广大读者和作者仔细甄别。