红小豆种皮色素提取及膜分离工艺研究
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(1. 黑龙江八一农垦大学食品学院,黑龙江 大庆 163319;2. 黑龙江省杂粮加工及质量安全工程技术研究中心,黑龙江 大庆 163319;3. 黑龙江省农产品加工工程技术研究中心,黑龙江 大庆 163319;4. 国家杂粮工程技术研究中心,黑龙江 大庆 163319)

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金丽梅(1972—),女,黑龙江八一农垦大学教授,博士。E-mail:jilinwa@126.com

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黑龙江省“杂粮生产与加工”优势特色学科资助项目(编号:黑教联\[2018\]4号);黑龙江八一农垦大学“三横三纵”平台支持计划项目(编号:PTJH201904)


Extraction and membrane separation of pigment from seed coat of red adzuki bean
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(1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China; 2. Heilongjiang Engineering Research Center for Coarse Cereals Processing and Quality Safety, Daqing, Heilongjiang 163319, China; 3. Agri-Food Processing and Engineering Technology Research Center of Heilongjiang Province, Daqing, Heilongjiang 163319, China; 4. National Coarse Cereals Engineering Research Center, Daqing, Heilongjiang 163319, China)

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    摘要:

    以水为溶剂对红小豆种皮中的红色素进行浸提,采用压力驱动的膜技术(微滤、纳滤等)优化红小豆种皮色素分离工艺。结果表明,红小豆种皮色素的最佳提取工艺为料液比(m红小豆种皮∶V)1∶30 (g/mL)、浸提温度90 ℃、浸提时间90 min,此时浸提液的色价为7.53;优化的膜分离工艺为微滤(0.22 μm)和纳滤膜(NF245)相结合,微滤压力0.075 MPa,膜通量12.47 L/(m2·h),色素透过率为87.16%;纳滤操作的最佳压力为0.3 MPa,稳定通量为16.06 L/(m2·h),截留液中色素质量浓度提高至810.19 mg/L,总酚、糖和蛋白质浓度进一步降低。纯化后的红豆皮花色苷冻干粉中花色苷含量为(145.80±0.17) mg/g,色价为58.08±0.09,为纯化前的2.41倍。

    Abstract:

    The red pigment from the seed coats of red adzuki beans was extracted by soaking in water and separated by the pressure-driven membrane technology (microfiltration, nanofiltration, etc.), and the separation processes were also optimized. The results showed that the optimal extraction conditions of pigment from seed coats were as follows: the ratio of material to liquid (mass of seed coats: volume of water) at 1∶30 (g/mL), extraction temperature 90 ℃, extraction time 90 min, and the color value of the extracts reached 7.53. The optimized membrane separation process was the combination of microfiltration (0.22 μm) and nanofiltration membrane (NF245), with the microfiltration pressure at 0.075 MPa the membrane flux speedat 12.47 L/(m2·h), and the pigment transmission rate at 87.16%; the optimal pressure for NF was 0.3 MPa, and the stable flux was 16.06 L/(m2·h). The content of pigment in the NF concentrate increased to 810.19 mg/L and the concentrations of total phenol, total sugar and protein were further reduced. The content of anthocyanin in the purified anthocyanin freeze-dried powder was (145.80±0.17) mg/g. The color value of the purified anthocyanin was 58.08±0.09, which was 2.41 times of that before purification.

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金丽梅,隋世有,任梦雅,等.红小豆种皮色素提取及膜分离工艺研究[J].食品与机械,2021,37(5):149-155.
JINLimei, SUIShiyou, RENMengya, et al. Extraction and membrane separation of pigment from seed coat of red adzuki bean[J]. Food & Machinery,2021,37(5):149-155.

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  • 收稿日期:2020-09-02
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  • 在线发布日期: 2023-02-15
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