In this research, vacuum freeze-drying, constant temperature of 40 ℃，constant temperature of 60 ℃, constant temperature of 100 ℃, constant temperature of 120 ℃, sun drying and variable temperature drying with air energy were used to dry Poria cocos, and the effects of different drying methods were studied on mycelium microscopic characteristics, water-soluble polysaccharide content, water-soluble protein content, alcohol-soluble protein content and antioxidant activity of Poria cocos. Results: The Poria cocos mycelium of variable temperature drying with air energy and sun drying were loosely arranged, and the Poria cocos mycelium of constant temperature drying was in a bond state. The antioxidant activity was the highest in variable temperature drying with air energy. No significant change in water-soluble polysaccharide content, highly significantly higher in water-soluble protein content (P＜0.01) and significantly decreased (P＜0.05) in alcohol-soluble protein, which was found in variable temperature drying with air energy. Comprehensive analysis, variable temperature drying with air energy was the best Poria cocos drying method, which can solve the demand of non-sulfur processing industrialization of Poria cocos.