The residue of Passiflora edulis Sims leaves after hot water extraction was used as material. Polysaccharide was extracted from the residue by using NaOH alkaline solution, followed by separation of ion-exchange chromatography. The physicochemical properties of polysaccharide were determined and analyzed by IR, HPLC and gel permeation chromatography, and then its hypoglycemic and anticoagulant were studied. The results showed that APEL was separated by DEAE-52 cellulose ion-exchange chromatography to obtain the APEL-1, APEL-2, APEL-3, the APEL and its separated components all have typical characteristic peaks of polysaccharides, mainly composed of arabinose, glucose, galactose, xylose, rhamnose, glucuronic acid. The molecular weights of APEL, APEL-1, APEL-2 and APEL-3 were 4.277×10⁶, 4.276×10⁶, 4.203×10⁶, 4.111×10⁶ Da. The APEL and its components could significantly inhibit α-amylase and α-glucosidase, and prolong the activated partial thromboplastin time, prothrombin time and thrombin time, showing obvious hypoglycemic and anticoagulant activities in vitro.