Using the principle of enzyme-linked immunosorbent assay, a method for quantitative determination of carbendazim residues in fruits, vegetables, tea, and tobacco was established. First, the carbendazim hapten was prepared through acid-base neutralization chemical reaction, and then the anti-carbendazim monoclonal antibody was screened out by immunizing mice to prepare the enzyme-labeled goat anti-mouse anti-antibody, and finally the prepared anti-carbendazim Monoclonal antibodies and enzyme-labeled goat anti-mouse anti-antibodies were used in the development of enzyme-linked ELISA kits. The results showed that within the linear range of 0.1~8.1 μg/L, the target carbendazim had a good linear relationship, and the correlation coefficient (R²) could reach 0.995. The recoveries of the kits at the addition levels of 300, 600, and 1 200 μg/kg were 76.0%~102.2%, and the relative standard deviations were all less than 10%. The kit detected carbendazim in tobacco leaves, apples, cabbage, and tea samples, and the detection limits were 266.3, 421.0, 349.1, and 484.4 μg/kg (n=20). This method was a rapid detection method and suitable for the determination of carbendazim residues in fruits, vegetables, tea, and tobacco leaves.