超高效液相色谱—串联质谱法快速同时检测茶叶中7种香料
CSTR:
作者:
作者单位:

作者简介:

梁志森(1989—),男,广州检验检测认证集团有限公司工程师,博士。E-mail:522794556@qq.com

通讯作者:

中图分类号:

基金项目:

广州市市场监督管理局科技项目(编号:2020kj46)


An UHPLC-MS/MS method for rapid analysis of tea for the potential presence of illegally added flavorings
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    基于超高效液相色谱—串联质谱(UHPLC-MS/MS),建立了同时检测茶叶中7种可能非法添加的香料(茴香烯、香豆素、胡椒酚甲醚、指甲花醌、甲基丁香酚、蒲勒酮和反式肉桂醛)的定性、定量方法。茶叶样品经过乙腈—水混合溶剂提取,涡旋振荡,适当稀释并加入无水硫酸钠、十八烷基键合硅胶(C18)和N-丙基乙二胺(PSA)进行净化,涡旋离心后,上清液过膜待测。以Kinetex C18色谱柱分离,在多反应检测扫描(MRM)模式下经MS/MS进行基质外标法定量检测。试验结果表明,7种目标物在0.8~1 600.0 μg/L范围内呈良好线性,线性相关系数(R2)均大于0.999。方法的检出限为0.02~0.03 mg/kg,定量限为0.05~0.10 mg/kg,平均回收率为82.5%~102.5%,日内、日间精密度分别小于16%和18%。该方法快速、可靠、灵敏度高,用于检测市面流通的100个茶叶样品,结果检出了反式茴香脑、香豆素和胡椒酚甲醚等物质,最大浓度值分别为65.0,2.0,72.2 mg/kg。

    Abstract:

    A qualitative and quantitative method for simultaneous detection of 7 potential presence of illegally-added flavorings, including trans-anethole, coumarin, estragole, lawsone, methyleugenol, pulegone and trans-cinnamaldehyde in tea samples. Tea samples were extracted with acetonitrile-water served as a mixed solvent, subjected to whirlpool oscillation and then diluted properly. After that, anhydrous sodium sulfate, primary secondary amine (PSA) and octadecylsiyl silica gel (C18) were added to the solvent for purification. The centrifugation, the supernatant was filtered over a membrane and separated on a Kinetex C18 column. These 7 target compounds were determined by UHPLC-MS/MS in MRM mode, and quantified utilizing a matrix-matched external standard method. The results showed a good linearity from 0.8~1 600.0 μg/L and the value of the corresponding correlation coefficients (R2) were all larger than 0.999. The values of limit of detection were 0.02~0.03 mg/kg, and those of limit of quantification were 0.05~0.10 mg/kg, the average recoveries were 82.5%~102.5%, and the intra-day and inter-day RSD values were below 16% and 18%, respectively. A survey of 100 commercial tea samples was conducted, showing that trans-anethole, coumarin and estragole were present in green and black tea with maximum concentrations of 65.0, 2.0 and 72.2 mg/kg, respectively.

    参考文献
    相似文献
    引证文献
引用本文

梁志森,陈玉珍,周朗君.超高效液相色谱—串联质谱法快速同时检测茶叶中7种香料[J].食品与机械,2020,(11):51-57.
LIANG Zhi-sen, CHEN Yu-zhen, ZHOU Lang-jun. An UHPLC-MS/MS method for rapid analysis of tea for the potential presence of illegally added flavorings[J]. Food & Machinery,2020,(11):51-57.

复制
相关视频

分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2023-02-18
  • 出版日期:
文章二维码
×
《食品与机械》
友情提示
友情提示 一、 近日有不少作者反应我刊官网无法打开,是因为我刊网站正在升级,旧网站仍在百度搜索排名前列。请认准《食品与机械》唯一官方网址:http://www.ifoodmm.com/spyjx/home 唯一官方邮箱:foodmm@ifoodmm.com; 联系电话:0731-85258200,希望广大读者和作者仔细甄别。