Established a mouse model of hyperlipidemia. The male mice were divided into blank control group, low fat group and high fat group, and the low dose, medium dose, high dose of Noni wine. Serum concentrations of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), respiratory exchange ratio (RER), were measured after 90 days of feeding. The results showed that medium dose and high dose can effectively reduce the body weight or body weight gain of the obese mice, and the testis fat rate, renal fat rate or body fat rate decreased. The serum TC content, TG content and LDL-C in hyperlipidemia mice were significantly reduced, and the HDL-C was increased. Noni wine can promote fat digestion, increase calorie production and activity of mice, which indicated that Noni wine had hypolipidemic effect.