The nutrients and bioactive compounds in fruits of Psidium guajava L. cv. Pearl, collected from Hechun town in Zhanjiang, were measured and determined in order to lay the foundation for the further development and utilization of this species. Results showed that the contents of moisture, crude fats, crude fiber, total carbohydrates, fructose, glucose, sucrose and citric acid in fresh fruits of P. guajava L. cv. Pearl were (88.98±0.04) g/100 g, (0.11±0.01) g/100 g, (2.27±0.01) g/100 g, (5.78±0.02) g/100 g, (22.00±0.20) mg/g, (22.05±0.25) mg/g, (12.45±0.15) mg/g, and (1.68±0.02), respectively. The content of crude protein was (0.78±0.01) g/100 g, and 16 kinds of amino acids including 7 essential amino acids were identified in P. guajava L. cv. Pearl fruits. The content of essential amino acids was 1 910.32 mg/kg, and the ratios of that to the total contents of amino acids and non-essential amino acids were 42.02%, 0.72, respectively. The content of ash was (0.50±0.05) g/100 g, and K, Ca and Fe were abundant in the fresh fruits. Furthermore, the results indicated that contents of water-soluble polysaccharides, polyphenols, flavonoids and vitamin C were (5.68±0.02) g/100 g, (180.84±0.15) mg/100 g, (193.58±0.01) mg/100 g, and (94.02±0.02) mg/100 g, respectively. Therefore, the nutritional and active ingredients in P. guajava L. cv. Pearl were abundant, which indicated the fruit could be widely used in food field.