气相色谱—串联质谱结合固相萃取技术同时测定番茄和黄瓜中10种杀菌剂
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张瑶,女,宁夏食品检测研究院工程师,硕士

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宁夏自然基金项目(编号:2019AAC03057);宁夏青年科技人才托举工程项目(编号:TJGC065);宁夏大学自然基金项目(编号:ZR18013)


Simultaneous determination of 10 kinds of fungicides in tomatoes and cucumbers by GC-MS/MS combined with solid phase extraction
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    摘要:

    建立了气相色谱—串联质谱(GC-MS/MS)测定蔬菜中啶氧菌酯、E-苯氧菌酯、醚菌酯等10种杀菌剂残留的检测方法。采用乙酸乙酯与环己烷的混合液对样品进行提取,优选出氨基固相萃取柱的净化方法,采用多反应监测(MRM)检测,外标法定量。结果表明,10种蔬菜杀菌剂药在15~500 ng/mL的质量浓度范围内线性关系良好,相关系数均大于0.999 1,方法检出限为0.1~0.5 μg/kg。实际样品加标回收率为84.7%~117.0%,相对标准偏差 (n=6)为0.4%~4.7%,该方法适用于蔬菜中多种杀菌剂的同时检测。

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    A method for the simultaneous determination of 10 fungicides residues in vegetables by gas chromatography- mass spectrometer (GC-MS/MS) was established. The samples were extracted by ethyl acetate cyclohexane, and the aminosolid phase extraction column purification was preferred. Which were detected by multi-reaction monitoring (MRM), and quantified by external standard method. The results showed that the linear relationship of 10 fungicides in the mass concentration range of 15~500 ng/mL was good, and the correlation coefficient was all greater than 0.999 1, with the detection limit of 0.1~0.5 μg/kg. The actual recoveries of the samples were 84.7%~117.0%, and the relative standard deviation (n=6) was 0.4%~4.7%. This method is suitable for the simultaneous detection of a variety of fungicides in vegetables.

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张瑶,吴龙国,马桂娟,等.气相色谱—串联质谱结合固相萃取技术同时测定番茄和黄瓜中10种杀菌剂[J].食品与机械,2020,(7):67-71.
ZHANG Yao, WU Long-guo, MA Gui-juan, et al. Simultaneous determination of 10 kinds of fungicides in tomatoes and cucumbers by GC-MS/MS combined with solid phase extraction[J]. Food & Machinery,2020,(7):67-71.

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  • 在线发布日期: 2023-02-17
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