Abstract:To investigate the antioxidant activity of different polar extracts from the stems of Ficus tikoua Bur. (FTB), the main chemical components of the highly active polar extracts were screened and identified. Petroleum ether, dichloromethane, ethyl acetate, n-butanol extract and water extract of the remaining upper layer were obtained by systematic solvent method. The antioxidant activities of different polar extracts were evaluated by the scavenging ability of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis-(3-ethylbenzothiazole-6-sulfonic acid) diammonium salt (ABTS) and total reducing ability (TRPA). HPLC-DAD-ESI/MSn-DPPH was used to quickly screen and identify the antioxidant characteristic components of higher polar parts from FTB stems. The results showed that the n-butanol extract had strong antioxidant activity (P<0.05). With the concentration increasing, there is a significant dose-dependent effect. The IC50 of n-butanol extract to DPPH and ABTS were (23.28±0.21) μg/mL and (76.30±1.13) μg/mL, respectively. Three compounds were identified to be 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid and 4-O-caffeoylquinic acid through comprehensive analysis of HPLC spectra, MS spectra, literature data and reference standards.