In the present study, the Lapsang Souchong essential oil was prepared by using macroporous resin, during which the preparation process was optimized. Furthermore, the volatile aroma components and antioxidant activity of the product were analyzed. The results showed that the optimal process was using LX-8 macroporous resin as the adsorption resin, and the aromatic components were absorbed in a constant temperature shaker (25 ℃, 200 r/min) for 180 min. The ratio of resin wet weight and substrate was 1∶350 (g/mL). Subsequently, macroporous resin was desorbed by using 95% ethanol in a constant temperature shaker (25 ℃, 200 r/min) for 150 min. The ratio of resin wet weight to ethanol solution was 1∶5 (g/mL). Under the control of this condition, the highest concentration of Lapsang Souchong essential oil was obtained. The aromatic components were concentrated 35 times, with the recovery rate approximate 51%. Gas chromatography-mass spectrometry (GC-MS) analysis indicated that most of the characteristic aroma components of Lapsang Souchong were adsorbed by LX-8 macroporous resin. Furthermore, the product was found to possess profound antioxidant activity. Its DPPH and ABTS free radical scavenging activity are 4 and 3 μg/mL ascorbic acid equivalent, respectively.