Indirect competition ELISA, high performance liquid chromatography and other techniques were used to study the sensitization and structural changes of glycosylated pollock albumin (PV) during simulated digestion in vitro. The results showed that the IgE / IgG binding capacity of glycosylated PV was significantly reduced after digestion, reached the lowest after 2 hours of gastrointestinal digestion, then slightly increased after 3 hours. Peptides with a molecular weight ＜ 500 Da produced by pepsin digestion under the action of trypsin re-aggregate, and the digestion products all had new emission peaks with red shifting. The fluorescence intensity of the emission peaks also decreased first and then increased. The results indicated that the change of PV sensitization was closely related to the change of its structure. The combination of glycosylation modification and simulated human gastrointestinal tract digestion could effectively reduce the protein sensitization.