47 isolates with obvious calcium dissolving circle were screened from fermented grains for light-Baijiu brewing by MRS calcium carbonate medium. Through physiological and biochemical characteristics and 16S rDNA sequences, it was found that the closed lactic acid bacteria strains were 20 strains Lactobacillus brevis, 16 strains L. plantarum, 7 strains L. buchneri, and 4 strains L. pentosus, respectively. By turbidimetric method, the autolysis of 47 strains of lactic acid bacteria was determined, and their autolysis ranged from 5.01%~29.27%. There was no correlation between the autosolubility and species, showing strains specificity. The autolysis characteristic of the 9 strains with high autolysis, MC30-2, LB27, YP15-1, SL25-1, SL33, MC46-2, YP49-1 and LB60, were analyzed. The results showed that the autolysis of most strains increased with the increase of environmental temperature and pH value.