碱诱导鸭蛋蛋清凝胶特性的变化规律
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凌子庭,男,华南农业大学在读硕士研究生

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河南省科技攻关项目(编号:182102310060);河南省重大科技专项项目(编号:161100110600);中国博士后科学基金(编号:2018M632767);河南省博士后科研项目(编号:001801021);河南省青年人才托举工程项目(编号:2018HYTP008);河南科技学院高层次人才引进项目(编号:2015015);河南科技学院重大科研培育项目(编号:2016ZD03)


Changes of gel properties during heating of duck albumen induced by alkali
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    摘要:

    以优选出添加0.50% NaOH的鸭蛋蛋清凝胶为研究对象,对质构、持水性、pH、色泽、蛋白质电泳(SDS-PAGE)及网络蛋白等指标进行测定,研究65~95 ℃范围内加热温度对碱诱导鸭蛋蛋清凝胶特性的影响。结果发现:凝胶的坚实度由加热前的68.88 g上升至最大值652.35 g(70 ℃),上升了8.47倍;弹性则由加热前的45.20%上升至95 ℃的77.50%;凝胶持水性的最大值为89.02%(75 ℃),95 ℃时下降至79.03%;凝胶的pH值由加热前的11.18下降至95 ℃的10.78,褐变程度随温度升高而加深。经65 ℃以上加热后,蛋白质发生聚集,在电泳图谱上出现分子量>97.4 kDa的新条带;网络蛋白含量在75 ℃达到最大值8.15%,后因热分解在95 ℃下降至7.21%。综合评判,70 ℃加热添加0.50% NaOH的蛋清可获得具有优良质构特性的碱诱导鸭蛋蛋清凝胶。

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    In this study, the effects of heating temperature on the gelation properties of duck albumen with 0.50% NaOH were studied by measuring the texture, water-holding capacity, pH, color, SDS-PAGE and net-work protein of duck albumen in the range of 65~95 ℃. The results showed that the firmness of the gel increased from 68.88 g to the maximum 652.35 g (70 ℃) after heating, with an increase of 8.47 times. Moreover, the elasticity increased from 45.20% to 77.5% as heated to 95 ℃. The water-holding capacity of the alkali-induced duck albumen gel increased to a maximum of 89.02% at 75 ℃, and then decreased to 79.03% at 95 ℃. The gel pH value decreased from 11.18 to 10.78 ℃ after heated to 95 ℃. The Browning degree of albumen deepened with the increase of temperature. After heating above 65 ℃, protein aggregation occurred and a new band with a molecular weight greater than 97.4 kDa was found in the electrophoresis pattern. Net-work protein content reached the maximum value of 8.15% at 75 ℃, and then decreased to 7.21% at 95 ℃ due to thermal decomposition. In conclusion, the alkali-induced gel of 0.50% NaOH with excellent texture characteristics could be obtained by heating at 70 ℃.

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凌子庭,蒋爱民,周佺,等.碱诱导鸭蛋蛋清凝胶特性的变化规律[J].食品与机械,2020,(3):1-6.
LING Zi-ting, JIANG Ai-min, ZHOU Quan, et al. Changes of gel properties during heating of duck albumen induced by alkali[J]. Food & Machinery,2020,(3):1-6.

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  • 在线发布日期: 2023-02-16
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