In order to study the biological function of GmWRKY86 gene in soybean on development and resistance against abiotic stress, PCR products of its opening reading frame, ORF) were used to clone GmWRKY86 gene, and the gene expressions in different tissues were detected by using real-time fluorescence quantitative PCR (qRT-PCR). Sequence analysis showed that the GmWRKY86 gene can encode 523 amino acids with an isoelectric point of 8.15, a molecular weight predicted to be 56.82 kDa, and an open reading frame of 1 572 bp. GmWRKY86 protein has two highly conserved domains, and the GmWRKY86 gene contains three introns and four exons. Phylogenetic tree analysis indicated that the GmWRKY86 protein belongs to the same branch as the FvWRKY42 (Fragaria vesca) and VvWRKY2 (Vitis vinifera). qRT-PCR analysis showed that GmWRKY86 gene was expressed in all tissues examined, with the highest expression in flowers and the lowest expression in seeds, indicating that GmWRKY86 gene involves in the development of soybean flower organs.