Pepsin and trypsin complex enzymes were used for biomimetic enzymatic hydrolysis of Eupolyphaga steleophaga. In order to obtain the most active fibrinolytic activity of the soil aphid active peptide component F2, the product was subjected to desalting, ultrafiltration, nanofiltration, DA201-C macroporous adsorption resin, dextran gel G-25 chromatography, reverse high performance liquid chromatography and semi-preparative liquid chromatography. Moreover, its effects on hemorheology index, blood lipids and serum nitric oxide (NO), superoxide dismutase (SOD), tissue plasminogen activator (t-PA) and fibrin (FIB) content of rats with acute blood stasis were also studied. E. ste-leophaga active peptide component F2 could significantly reduce blood viscosity, hematocrit and platelet count (P＜0.05). Compared with the model group, the Eupolyphaga ste-leophaga active peptide component F2 can significantly reduce the content of triglyceride (TG), cholesterol (T-CHO), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in rat blood (P＜0.05). The levels of NO, SOD and t-PA in the blood of rats were significantly higher than those in the model group, and significant differences in the levels of FIB between the experimental group and the model group (P＜0.05) were also found. The E. ste-leophaga active peptide component F2 prepared by biomimetic enzymatic hydrolysis and purification had the functions of improving blood rheology index, reducing blood lipid levels and changing blood factor content in acute blood stasis model rats.