A method for rapid determination of aflatoxin B1 in grain based on QD-Infinity immunofluorescence was established with QD-Infinity as fluorescent probe. In order to confirm the accuracy of this method, multiple methods were used to evaluate the uniformity of the test results of AFB₁ in grain which was determined by the QD-Infinity immunofluorescence method, high performance liquid chromatography and enzyme association immunoadsorption, respectively. Moreover the repeatability of the method and the difference between stations were investigated. The detection results of the superconducting point-immunofluorescence method for grain samples such as rice and corn were consistent with those of high performance liquid chromatography and enzyme association immunoadsorption, indicated that the method was accurate and reliable. There was no significant difference in the detection results when using two different instruments, which inclined a good repeatability of the method was good too.