The DNA of Fritillariae cirrhosae bulbus was extracted by alkali lysis. The effects of different proportion of lysate on DNA quality and electrophoretic success rate were investigated, which compared with the current methods. Results, The alkaline lysis buffer had the best effect on DNA extraction, which was made of 200 mmol/L NaCl, 5 mmol/L Mg₂Cl, 1% Triton X-100, 1% PVP, 1% SDS, 2 mmol/L EDTA and 0.1 mol/L Tris-HCl. The DNA electrophoretic bands extracted by this alkali lysate were in accordance with the current method. The method can save 15~20 minutes, and the cost of single sample testing can be reduced to 0.01 yuan. This method was used to identify 25 samples of Fritillariae cirrhosae bulbus on the market. The results showed that Fritillariae cirrhosae bulbus could be identified quickly.