Diabetic zebrafish model was established quickly by combination of 20 g/L glucose solution immersing and 10% cholesterol feeding for 20 days. The model established was used to evaluate the in vivo hypoglycemic activity of 3 000～100 Da AKEH. The effects of it on physicochemical indexes such as DPP-IV activity, glucose, triglyceride, cholesterol and expression levels of relative gene such as Insa, Glucagon and Pck1 of diabetic zebrafish were investigated. The results showed that the physicochemical index and blood glucose related gene expression level of the 1.35 g/L and 2.7 g/L concentrations were not significant difference. 3 000～100 Da AKEH of 5.4 g/L could be reduced the level of physicochemical index, significantly inhibited DPP-IV activity, the expression level of Glucagon and Pck1 gene and improved Insa expression level. Therefore, 3 000～100 Da AKEH has certain hypoglycemic effects.