大孔树脂纯化苜蓿叶蛋白肽的工艺优化

doi:10.13652/j.issn.1003-5788.2019.05.030

首页 > 过刊浏览>2019年第卷第5期 >167-171. DOI:10.13652/j.issn.1003-5788.2019.05.030

大孔树脂纯化苜蓿叶蛋白肽的工艺优化
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                        10.13652/j.issn.1003-5788.2019.05.030
                    
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作者单位:(1. 齐齐哈尔大学食品与生物工程学院，黑龙江 齐齐哈尔 161006;2. 黑龙江省农科院畜牧兽医分院，黑龙江 齐齐哈尔 161005)
作者简介:许英一（1971—），女，齐齐哈尔大学教授，硕士。E-mail:wyxyy-1@163.com
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基金项目:黑龙江省教育厅基本业务专项（编号：LTSW201724）；黑龙江省自然科学基金项目（编号：C201456）

Optimization on purification technology of alfalfa leaf protein peptide bymacroporous adsoption resin

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(1. College of Food and Bioengineering, Qiqihar University, Qiqihar, Heilongjiang 161006, China; 2. Animal Husbandry and Veterinary Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar, Heilongjiang 161005, China)

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摘要:

通过对比6种大孔树脂对苜蓿叶蛋白肽吸附效果，筛选最佳大孔吸附树脂，并研究其对苜蓿叶蛋白肽的吸附—解吸特性。试验结果表明，纯化苜蓿叶蛋白肽的最适大孔吸附树脂为DA201-C型，最佳工艺条件为：上样流速0.5 mL/min，上样浓度10 mg/mL，75%乙醇溶液为洗脱剂，洗脱流速0.5 mL/min，洗脱体积200 mL。在该条件下苜蓿叶蛋白肽含量较最初提高了46.95%，糖含量降低了81.88%，盐和其他杂质含量降低了70.97%。DA201-C型大孔吸附树脂对苜蓿叶蛋白肽有较好的纯化效果。

Abstract:

The best macroporous adsorption resin was selected by comparing the adsorption effects of six macroporous adsorption resins on Alfalfa Leaf Protein (ALPP), and the adsorption-desorption characteristics of ALPP were studied. The results showed that the best type of macroporous adsorption resin for purifying ALPP was DA201-C. The optimum conditions were as follows: flow rate of sampling was 0.5 mL/min, concentration of sampling was 10 mg/mL, 75% ethanol solution was an eluent, the elution flow rate was 0.5 mL/min and the elution volume was 200 mL. Under these conditions, content of ALPP inceased by 46.95%, content of sugar decreased by 81.88%, content of salt and other impurities decreased by 70.97%. DA201-C macroporous adsorption resin had better purification effect on ALPP.

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许英一,吴红艳,王宇,等.大孔树脂纯化苜蓿叶蛋白肽的工艺优化[J].食品与机械,2019,(5):167-171.
XUYingyi, WUHongyan, WANGYu, et al. Optimization on purification technology of alfalfa leaf protein peptide bymacroporous adsoption resin[J]. Food & Machinery,2019,(5):167-171.

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收稿日期:2019-01-18
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在线发布日期: 2022-11-26
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