Aquatic products is easily deteriorated，and microorganism is the main factor that leads to the deterioration. Predictive microbiology can predict the growth of spoilage organisms in aquatic products by building mathematical models, which help us predict the shelf-life of aquatic products. This paper briefly describes the meaning of predictive microbiology primary model, secondary model and tertiary model, enumerates the applications of predictive microbiology in shelf-life, and discuss the problems of predictive microbiology in the shelf-life prediction, and its application prospect is also proposed.