Bee Pollen Soluble Dietary Fiber (BPSDF) was extracted from Lotus bee pollen by cellulase enzymatic extraction, acid solution extraction and alkali solution extraction. The effects of three extraction methods on the polyphenols contents and the growth of harmful bacteria and beneficial bacteria were studied.The results showed that the polyphenols in BPSDF were mainly in free form. The contents of free polyphenols in BPSDF were 81.16%, 80.34% and 69.59% respectively by cellulase enzymatic extraction, acid solution extraction and alkali solution extraction. The contents of free phenol and total polyphenols in BPSDF were relatively higher by cellulase enzymatic extraction,and the content of the binding polyphenols in BPSDF was relatively higher by alkaline solution extraction. The contents of free polyphenols, binding polyphenols and total polyphenols in BPSDF were significantly different with different extraction methods, but the effects on the constituents of free polyphenols and binding polyphenols in BPSDF were less. BPSDF by three extraction methods has good inhibitory effects on E. coli, Staphylococcus aureus and Candida sporogenes respectively,and BPSDF by cellulase enzymatic extraction has the better inhibitory effect.BPSDF by three extraction methods has certain promoting effects on Bifidobacterium and Lactobacillus acidophilus.The inhibition effects of BPSDF on the tested harmful bacteria were better than that of the tested beneficial bacteria.