Based on quenching effect of ochratoxin A on chemiluminescence (CL) reaction of luminol-NaIO4 system catalyzing by Tween 20, a novel flow-injection chemiluminescence method was developed for the rapid on-line determination of possible sudden pollution of ochratoxin A in drinking water. Under the optimum conditions of response surface experiment, the linear range for ochratoxin A was 0.006～1.000 μg/mL with a detection limit of 1.74×10^-3 μg/mL and recovery of 80.6%～92.4%. The relative standard deviation (RSD) was 1.68% by analyzing samples containing 0.02 μg/mL ochratoxin A (n=11). This method was founs convenient to operate, and couls be applied to the rapid emergency warning and real-time monitoring of ochratoxin A in drinking water.