Used moringa seed shell as adsorbent, investigated the effects of adsorption time, particle size, temperature, initial concentration of solution and pH on the adsorption of methylene blue. Datas were fitted by adsorption isotherm model and kinetic model. The samples were characterized by scanning electron microscopy and infrared spectroscopy. The results showed that under the condition of adding 10 g/L of moringa seed shell, the optimum adsorption conditions were adsorption time 1.5 h, particle size ＞80 mesh, temperature 25 ℃, methylene blue solution concentration 200 mg/L and pH＞5. The adsorption process followed the Langmuir model and the pseudo-second-order kinetic model. The maximum theoretical saturated adsorption capacity was 94.25 mg/g. The adsorption process was a common result of physical adsorption and chemisorption, and mainly by physical adsorption.