This study aimed to investigating the effects of the polyphenols from Areca seeds on young men’ s intestinal microbes in vitro through intermittent static anaerobic fermentation technology. The feces of 24 years healthy young men are taken as starter. The substrates of the control group (group C) and the experimental group (group P) with a purity of 74.09% were fermented in vitro at 2, 6, 12 and 24 h. pH value, polyphenol composition, short chain fatty acid (SCFAs) and microorganism index were measured by gas chromatography and high-throughput sequencing. The results showed that the polyphenols of the Areca-nut polyphenols had little effect on the change of pH in the intestinal microenvironment of young healthy men, but it reduced the content of SCFAs. In terms of intestinal flora, it could improve the species diversity level of its intestinal flora, reduce the relative abundance of Proteobacteria, and improve the relative abundance of Firmicutes. Among them, the largest increase is Faecalibacterium, while the relative abundance of Bacteroides and Bifidobacterium was also slightly increased. The above results showed that betel nut polyphenols could change the structure of intestinal flora, but no similar health effects was found.