By using the ham sausages currently sold in Chinese market as studying materials, upright light microscope and photography system were employed in treating the stained slices of ham sausages. Photographs observable by naked eyes were obtained and the pixels occupied by the stained area of proteins, fats and starches were measured respectively while their percentages accounted for the pixels of total observed area were subsequently calculated. Results analysis indicated that the percentage of the pixels which were occupied by the stained area of proteins, fats or starches accounted for the pixels of total observed area was positively correlated with their content in the ham sausage, respectively. While it also had positive linear correlation with the hedonic score of structure of ham sausages. Direct observation of photographs showed that the distribution of proteins, fats and starches in different grades of ham sausages was significantly different, respectively. Cluster analysis indicated that the percentage of the pixels which were occupied by the stained area of proteins and starches accounted for the pixels of total observed area as well as the hedonic score of structure of ham sausages well distinguished their categories. Therefore, microphotography is applicable to evaluation of quality of ham sausages.