Redryed tobacco in Xingyi, Guizhou was used as the material, and the effects of moisture, inoculum amount, fermentation temperature and time on the content of petroleum ether extracts were investigated by using solid fermentation of aroma producing yeast. Then, the fermentation conditions were optimized by using orthogonal test. The aroma and conventional chemical composition in tobacco under the optimal condition were determined. The results showed that ① the effective factors on the content of petroleum ether extracts were the order of inoculation amount ＞ fermentation temperature ＞ fermentation time ＞ tobacco moisture. The optimal fermentation process were tobacco moisture 30% inoculum amount 5 mL, fermentation temperature 30 ℃, fermentation time 2 d. The petroleum ether extract content was upto 8.45%. ② Under the optimal fermentation conditions, many kinds of tobacco aroma were newly increased, such as alcohol, farnesol, and β-cyclocitral, and neophytadiene, which of the content was increased upto 78.07 μg/g.