Oil samples were extracted with methanol, and BHA, BHT and TBHQ in food were detected by gas chromatography. Result: When the concentration of BHA, BHT and TBHQ was in the range of 0.01~0.20 mg/mL, all of the correlation coefficients of BHA, BHT and TBHQ had good linear relation with r>0.999, with the detection limits<0.5 μg/mL. The recovery rate of the method met the requirements of laboratory determination, and the standard deviation was between 1.07~3.10 and the relative standard deviation was less than 5%.