The polysaccharide was extracted by compound enzyme method from the Indian truffle. Through single factor and uniform design experiments, the effect on the yield of truffle polysaccharide were investigated, including the enzyme ratio, zymolytic time and temperature, enzyme dosage, material/ liquid ratio and pH value. According to U₁₀ (10⁸) experiment, the effects of six single factors as well as their interactions on the yield of polysaccharide were studied. The optimal process parameters were predicted and verified. The results showed that the best extraction conditions of truffle polysaccharide was as follows: dispase and cellulase ratio of 1∶7 （g/g）, zymolytic time 48 min, zymolytic temperature 41 ℃, enzyme dosage 3.6%, material/ liquid ratio 1∶29 (g/mL) and pH 4.8. The yield of polysaccharide was up to 14.50%, and the content of polysaccharide could reach 75.96%. The confirmatory tests proved that the optimal conditions were reliable and suitable for extracting truffle polysaccharides.