The eggshell-crack can be detected by the acoustic features of impact response signal in a non-destructive way. To collect and analyze the acoustic signals in real time, an eggshell crack detection system based on TMS320F28335 was designed. The acquisition of the responded audio signal was triggered by the external interrupt of DSP. By comparison of frequency spectrums between intact and cracked eggs, there is a significant difference in the audio signal spectrum. The frequency of the second formant of the intact egg spectrum is concentrated at about 6 890 Hz, while the frequency of the second formant of the cracked egg has no obvious regularity. In conclusion, the system can meet the requirements of detection and provide a hardware platform for the further research of eggshell crack detection algorithms.