姜多糖提取方法工艺优化及分析
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(中国药科大学工学院,江苏 南京 211198)

作者简介:

廖登未, 男,中国药科大学在读硕士研究生。

通讯作者:

陈贵堂(1977—), 男,中国药科大学副教授,博士。E-mail: caucgt@163.com

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国家重点研发计划“现代食品加工及粮食收储运技术与装备”重点专项(编号:2017YFD0400203)


Optimization of polysaccharides extractedand analysis from Zingiber officinale rosc
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(College of Engineering, China Pharmaceutical University, Nanjing, Jiangsu 211198, China)

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    摘要:

    为探究提取方法对姜多糖提取效果的影响,采用热水浸提法、超声波冰浴提取法以及复合酶酶解法提取姜多糖,优化出各自最佳提取工艺条件,并对比多糖紫外光谱扫描以及姜渣透射电镜扫描图。结果表明:热水浸提法、超声波冰浴提取法以及复合酶酶解法3种提取工艺在最佳提取条件下得到的多糖提取率分别为(11.74±0.23)%,(7.00±0.04)%,(20.93±0.20)%,得率分别为(6.53±0.08)%,(2.56±0.11)%,(14.67±0.32)%,纯度分别为(86.53±0.20)%,(59.84±0.06)%,(73.59±0.19)%;对比3种不同方法可知,复合酶解法姜多糖提取率和得率最高,而在纯度方面,热水浸提法优于复合酶解法和超声波冰浴提取法。结合姜渣透射电镜图发现,不同提取方法对姜细胞的破坏程度不同,从而造成多糖的提取率和多糖得率不同。

    Abstract:

    The polysaccharides from Zingiber officinale Rosc were extracted by hot water extraction method, ultrasonic ice bath extraction method and complex enzymatic hydrolysis method. The effects of different methods on the extraction were investigated by the UV spectra image and the scanning electron microscope image of ginger residue. The results showed that the polysaccharides extraction rates of the three different extraction processes under optimal extraction conditions were (11.74±0.23)%, (7.00±0.04)%, and (20.93±0.20)%, and the yields were (6.53±0.08)%, (2.56±0.11)%, and (14.67±0.32)%, the purity was (86.53±0.20)%, (59.84±0.06)%, and (73.59±0.19)%. Comparing with three different methods, the enzymatic hydrolysis method had the highest extraction rate and yield of Zingiber officinale Rosc polysaccharides, but in terms of purity, the hot water extraction method was the best. According to the TEM image of ginger residue, found different extraction methods had different degrees of damage to Zingiber officinale Rosc cells, resulting in different extraction rates and yields of polysaccharides.

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引用本文

廖登未,黄德春,程抒劼,等.姜多糖提取方法工艺优化及分析[J].食品与机械,2018,34(9):152-156.
LIAODengwei, HUANGDechun, CHENGShujie, et al. Optimization of polysaccharides extractedand analysis from Zingiber officinale rosc[J]. Food & Machinery,2018,34(9):152-156.

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  • 收稿日期:2018-04-01
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  • 在线发布日期: 2023-03-17
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