基于不同检测器高效液相色谱法测定食品中糖醇的研究
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(湖南省食品质量监督检验研究院,湖南 长沙 410000)

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向俊(1984—),女,湖南省食品质量监督检验研究院工程师,硕士。E-mail:105224640@qq.com

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Analysis and assessment of two kinds of high performance liquid chromatography methods to detect xylitol, sorbitol and maltitol in foods
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(Hunan Institute of Food Quality Supervision Inspection and Research, Changsha, Hunan 410000, China)

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    摘要:

    分析比较了口香糖、饮料样品中3种糖醇的2种测定方法。样品经水直接提取后一分为二,一份用配有示差折光检测器的高效液相色谱法检测,另一份用配有蒸发光散射检测器的高效液相色谱法检测。高效液相色谱法—示差折光检测法(HPLC-DRID)测定的木糖醇、山梨醇和麦芽糖醇的检出限(RSN=3)均为0.037 5 g/100 g,相关系数为0.999 21~0.999 29,加标回收率为91.5%~97.3%;高效液相色谱法—蒸发光散射检测法(HPLC-ELSD)测定的木糖醇、山梨醇和麦芽糖醇的检出限(RSN=3)为0.009 5~0.027 5 g/100 g,相关系数为0.999 3~0.999 6,加标回收率为91.2%~96.3%。结果表明:2种高效液相色谱法均能满足样品中3种糖醇的检测要求,而高效液相色谱法—蒸发光散射检测法的灵敏度要明显优于高效液相色谱法—示差折光检测法的,更适用于糖醇含量低的样品检测。

    Abstract:

    Two different methods for the determination of three kinds of sugar alcohols in bubble gum and beverage were analyzed and compared. Samples were extracted directly by water. Half part of the extracts was detected by high performance liquid chromatography with differential refractive index detection (HPLC-DRID), the rest part of the extracts was detected by high performance liquid chromatography with evaporative light-scattering detection (HPLC-ELSD). Limits of detection (LOD) of xylitol, sorbitol and maltitol determined by HPLC-DRID were 0.037 5 g/100 g, the correlation coefficients were in the range of 0.999 21~0.999 29, and the recovery rates of spiked samples were in the range of 91.5%~97.3%; LODs of xylitol, sorbitol and maltitol determined by HPLC-ELSD were in the range of 0.009 5~0.027 5 g/100 g, the correlation coefficients were in the range of 0.999 3~0.999 6, and the recovery rates of spiked samples were in the range of 90.9%~96.3%. All results showed that both of HPLC-DRID and HPLC-ELSD were the appropriate methods for the determination of three kinds of sugar alcohols in samples. Compared with HPLC-DRID, the HPLC-ELSD is a more optimal method for the sample with low sugar alcohols contents because of its high sensitivity in the test.

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向俊,王芳斌,荆辉华,等.基于不同检测器高效液相色谱法测定食品中糖醇的研究[J].食品与机械,2018,34(9):92-95.
XIANGJun, WANGFangbin, JINGHuihua, et al. Analysis and assessment of two kinds of high performance liquid chromatography methods to detect xylitol, sorbitol and maltitol in foods[J]. Food & Machinery,2018,34(9):92-95.

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  • 收稿日期:2018-04-08
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  • 在线发布日期: 2023-03-17
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