In order to realize the stable quality and large-scale production of Guizhou Sansui characteristic bacteriallobster sauce.Pure cultivar and mixed bacillus fermentation was carried out to strengthen the dominantstrainsbased on the preservation of the original flavors. Thirteen strains were isolated and screened from traditional production techniques and inoculated into soybeans. Preliminary screen and rescreen by using sensory evaluation and determination of protease activity as indicators,five strains of dominant strains were selected for physiological and biochemical experiments. Finally, three strains of B, F and J were selected for 16Sr DNA sequencing ，and establishingphylogenetic trees. The results showed that strains of B, F and J belong to the subspecies of Bacillus, which are the dominant strains of the characteristic bacteria-type lobster saucein Guizhou Sansui. They can lay the foundation for the preparation of the compound inoculants for lobster sauce fermentation.