蒸汽爆破提取牛膝多糖工艺优化及抗氧化性研究
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(1. 河南科技大学食品与生物工程学院,河南 洛阳 471023; 2. 河南科技大学化工与制药学院,河南 洛阳 471023)

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易军鹏(1976—),男,河南科技大学副教授,博士。E-mail: yijunpeng@126.com

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河南省科技攻关计划(编号:182102110085)


Optimization of steam explosion pretreatment for polysaccharides extraction from Achyranthes bidentata radix and of its antioxidant activity evaluation
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(1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan 471023, China; 2. College of Chemical Engineering and Pharmacy, Henan University of Science and Technology, Luoyang, Henan 471023, China)

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    摘要:

    利用蒸汽爆破预处理牛膝提高多糖提取率。在单因素试验基础上,采用响应面分析法优化蒸汽爆破预处理工艺。以DPPH自由基清除率和O2-自由基清除率为指标,研究蒸汽爆破处理对牛膝多糖抗氧化活性的影响。结果表明,蒸汽爆破的最优工艺为维压时间63 s、预浸泡含水率11%、蒸汽爆破压力1.6 MPa,该条件下牛膝多糖得率为11.88%,是未处理的2倍左右。扫描电镜分析显示蒸汽爆破使牛膝表面断裂、出现空洞,可能是多糖提取率提高的原因。体外抗氧化试验表明,经汽爆破处理后,牛膝多糖抗氧化性显著增加,清除DPPH自由基能力的最大值由原来的77.9%增大至90.1%,O2-自由基清除率也提高到1.3倍左右。结合红外分析,可能是汽爆处理导致牛膝多糖中被纤维类包裹的一类多糖释放,增加了多糖的含量和种类。

    Abstract:

    Extraction yield of polysaccharides from Achyranthes bidentata Radix (AbR) was increased by steam explosion pretreatment. Based on the results of single-factor experiments, the response surface methodology was employed to further optimize the steam explosion pretreatment conditions. The effect of steam explosion pretreatment on the DPPH radical scavenging activity and O2- radical scavenging activity of Achyranthes bidentata Polysaccharides (AbPS) was evaluated. The optimum conditions was determined as follows: the water/AbR ratio 11%, steam explosion pressure, 1.6 MPa; residence time 63 s. After pretreatment under the optimum conditions, the extraction yield of polysaccharides from AbR was found to be 11.88%, which was twice as much as that from the untreated AbR.It maybe caused by the physical damage on the surface of AbR, including partial rupture with deep longitudinal cracks and micro-holes, due to the pretreatment of steam explosion, which can be seen from the SEM micro photographs. Furthermore, the antioxidant activity of AbPS was enhanced strongly after steam explosion pretreatment. The DPPH radical scavenging activity of AbPS increased from 77.9% to 90.1%, while the O2- radical scavenging activity of AbPS was around 1.3 times as much as that from untreated AbR.

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易军鹏,王赛,李欣,等.蒸汽爆破提取牛膝多糖工艺优化及抗氧化性研究[J].食品与机械,2018,34(6):145-151.
YIJunpeng, WANGSai, LIXin, et al. Optimization of steam explosion pretreatment for polysaccharides extraction from Achyranthes bidentata radix and of its antioxidant activity evaluation[J]. Food & Machinery,2018,34(6):145-151.

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  • 收稿日期:2018-02-15
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  • 在线发布日期: 2023-03-17
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